Gram Staining and Distilled Water Essay Example for Free

Gram Staining and Distilled Water Essay The purpose of this experiment was to learn how to properly perform a gram stain and to also understand how gram staining can help identify either gram-positive or gram-negative organisms in the laboratory. The materials needed for this experiment included: two microorganisms (Staphylococcus aureus and Pseudomonas fluorescens) in a slant agar, an inoculating loop, a microscope slide, distilled water, a Bunsen burner, four dyes (crystal violet, Grams iodine, ethyl alcohol, and safranin), bibulous paper, and a dye tray. The method for this experiment was as follows: 1) Cleaned the microscope slide with both soap and water and then dried 2) Flamed the inoculating loop with the Bunsen burner and then applied two small drops of water to each side (horizontally) of the slide 3) Flamed the inoculating loop again, allowed it to cool, and obtained a small sample of the first organism 4) Mixed the organisms on the inoculating loop with one of the drops of the water on the slide and spread to a nickel-sized drop 5) Repeated steps 3 and 4 for the other organism using the second water drop on the slide 6) Allowed the samples to dry and then heat fixed both samples. Applied the crystal violet dye, let it soak for 20 seconds, and then washed the dye away with distilled water off into the dye tray 8) Applied the Grams iodine and let it soak for a minute 9) Washed it off with alcohol for 20 seconds (or until the dye stopped running), and then immediately washed with the distilled water 10) Applied the counter-stain, safranin, and let it soak for a minute. 11) Washed with distilled water after and gently blotted it dry with bibulous paper 12) Observed both microorganisms on slide under the microscope Two different results were observed under the microscope. The first one was purple colored cocci, and the picture of the slide is on the following page. The second one revealed pink-colored rods, and the image is on the page following the first microorganisms. The results of the experiment came out as expected. There didnt seem to be any flaws in the experiment, since the colors were distinctly different. The purple cocci were identified as the Staphylococcus aureus. Because the violet dye penetrated the membrane and stained it, it was recognized as a gram-positive organism. The pink rods were Pseudomonas florescens. It was discovered to be a gram-negative organism because the organism didnt stain from the crystal violet. Since everything was easily identifiable, the experiment seemed to have gone as planned.

Yahoo Vs. Lycos Essay example -- essays research papers fc

Yahoo! Vs. Lycos When searching on the Internet, one may find it difficult sometimes to know where to start. With the seemingly limitless amount of information, one should use the resource suitable for the searcher's needs and tastes. Comparing different factors like databases, directory types, strengths and weaknesses of two search engines, such as Yahoo! and Lycos, can provide an advantage to someone looking for a starting block. To start with, one of the oldest search engines on the web, Lycos continues to thrive mainly by providing a mix of features. As the trend with the other major search tools, Lycos consists of a conglomeration of databases, online services, and other Internet properties. In terms of general searching, Lycos search engine displays the new advanced search with FAST, which means searching one of the largest Web page databases. Within the last year, Lycos made its main directory a version of the Open Directory Project. With a goal of this directory reaching the status of the most comprehensive directory of the web, and with over 1.8 million listings in 283,798 categories, it includes more pages than Yahoo!. Similar to Yahoo!, Lycos has searchable listings by category and the sites are reviewed and categorized by editors. Humans perform better at making these kinds of decisions than computers, so results will often eclipse with the other search engines. While Yahoo! can take months to take submissions of new Web sites, sites usually start in the Open Dir...

Managerial Communications Essay

Hynes introduces a calculated approach to managerial communication by dissecting it into three separate, yet mutually dependent functions. Hynes believes that with these approaches, management and employees alike can learn to adapt to one another to create an effective work force. The first layer is based on the idea that an employer and his employees can create a positive work atmosphere with the communication climate they set with one another. If a manager and his employees have an open and trusting relationship, it makes communications much easier for the both of them, regardless of their status. By allowing employees to contribute their thoughts and opinions without the fear of backlash, managers will see better job performance within their workers. Employers can often create an open communication climate through regular team building meetings and workshops. Communication climate also challenges managers to do their part by listening to their employees’ feedback. In order for effective communication to occur, an employee should feel comfortable in their work atmosphere and fully believe that their manager will take their words into deep consideration. Founder and President of Pillar Consulting LLC , Joelle K. Jay, PhD states, â€Å"On a personal level, people feel acknowledged when others validate their feelings. Managers who ignore feelings can create distance between themselves and their employees, eroding the relationship and ultimately affecting the working environment (Jay, â€Å"Communicate Well: Five Strategies To Enhance Your Managerial Communication Skills†). † From that statement, managers must also learn the boundaries within successful communication. In Hynes’ text, he also states that, â€Å"a positive climate is fragile†¦after only one or two critical errors, a positive environment can quickly change to one of distrust and closed communication, making future communication more difficult† (28). Managers must also learn to face the challenges of workers who become too comfortable in their environment. For example, Manager A and his Employee B, may have a friendly and casual relationship—one in which they may feel so comfortable that they talk about their personal and/or family business with one another. This is good in the sense that Employee B learns to trust Manager A and feels he can chat comfortably about his feelings toward his job. Now, if Employee A begins to feel so comfortable that Manager A is finding consistent errors within Employee B’s work, it is only right for Manager A to step in and confront him about the matter. Depending upon the choice of words used by Manager A and how Employee B feels about the situation, it can change the communication climate. One bad meeting can leave Employee B feeling angry with Manager A. He may no longer wish to have such a friendly demeanor towards him in the future. This can cause a strain on their communication with each other in the future. Another way to open a communication climate is through organizational culture. In an age of such advanced technology, many businesses no longer feel the need to speak to a person directly, either face-to-face or over the phone. The use of email has become a strong tool in business as it allows users to speak to each other without having to schedule a set time for both parties to meet only to say a few words. From the previous example, Manager A may be in a business that is highly dependent on email to speak to his employees in order to give clear, direct orders. Employee B may have previously come from an organization where meeting face-to-face was the ideal way to speak to anyone—management or employees alike. If Manager A decided it would be easier to email Employee B of the mistakes that he’s been making, Employee B may take that as a sign of disrespect. Employee B may not understand the tone that Manager A is reflecting in the email, leaving Employee B with the notion that Manager A couldn’t take time out of his schedule to speak with him directly. Their different perspectives of organizational culture could cause their gap in communication to widen tremendously. Organizational culture can help managers to better understand Hynes’ second layer in his approach to strategic communication. As the sender, Manager A’s personal characteristics can greatly affect the way he communicates with his employees. Before speaking with Employee B about his mistakes, Manager A must first reflect on what he will say to Employee B and how he will say it to him. Efficient communication can be the determining factor for communications in the future. While an employee only has to adjust his communication for his manager, a manager will need to find a way to relay his message to each employee in the most comfortable and appropriate manner they deem fit. The manager must constantly adjust this speech for each person that he meets with to ensure a satisfactory outcome. Though employees must mainly focus on how to communicate with management, there are various factors they must also take into consideration. How an employee takes in what an authority figure is saying to them greatly depends on their closeness with management, how they feel about the subject, concern for the subject, their current mindset, and their differences in position. As a receiver, an employee can choose how to accept a message being given to them by their employer. In the previous example between Manager A and Employee B, it was said that Manager A would have to speak with Employee B about the mistakes he’s been making in his work. Since both parties already have a close relationship, Employee B will be more open to hearing what Manager A has to say. Employee B may have had a bad morning and doesn’t want to talk to anyone, let alone hear what Manager A has to say about his errors. By having a platonic relationship with his employee, Manager A is at an advantage because Employee B will respect him for their friendship. Employee B will be more willing to take Manager A’s words into consideration and change his future actions. Managers must also be mindful of the language they use when speaking with their employees. If they are knowingly using jargon that their employee will not understand, communicating properly will be a difficult task. Not all conversations between employers and employees are ones that criticize their work. Before speaking to their employees, whether in a group setting or a one-on-one conference, a manager must first carefully examine the topic. He must verify that the subject matter would be something that is beneficial to the work force, or something that is unnecessary to building the work morale. In Hynes’ third layer, it is learned that in order to measure how successful a communication approach is, managers must also consider how to get their message across to their employees. As stated before, Manager A may want to email Employee B on the subject of his work. Before doing so, Manager A must fully analyze the words he will be saying to Employee B and recognize if the matter is something that may be better said in person. Also, in Employee B’s organizational culture, he may not be acclimated to receiving the critique of his work in writing. By speaking to Employee B personally, Manager A has the chance to show him that he respects him by meeting him face-to-face. To ensure a message is clearly stated to employees, managers must also take the necessary steps to find a comfortable place and time for them to meet. If the place of employment is one that deals with much noise such as a construction site, managers should take his directed employees to a comfortable place away from many outside distractions. Managers must also consider the amount of time it may take to speak to his workers. On a construction site, more time wasted can also lead to more money wasted. Managers must carefully plan their speech in an amount of time that does not interfere with the actual workload. By using these tactics laid out by Geraldine E. Hynes, managers can begin to set up their own communication strategies to ensure future success. Managers can compare and use these strategies as a baseline to communicating efficiently.

What Is A Long Lasting Tradition Essay - 866 Words

Long Lasting Tradition We walk in and the atmosphere fills me with joy; I hear children yelling on roller coasters, the amazing aroma of greasy, fried pork rinds, seeing smiles everywhere, it just sets the mood of having a great day. My family and I are at the famous theme park, Silver Dollar City. We have had a lasting tradition of visiting, every Christmas holiday and enjoying the sights and sounds of this amazing place. Our first stop is the store, Christmas Hollow, where Christmas is everywhere. Bright lights are throughout the whole store, while the smell of cinnamon and peppermint fill the air. I walk very carefully; not wanting to break any of the very fragile glass all around me. Finally, my favorite item, the nutcrackers;†¦show more content†¦Thunderation is one of my favorite rides in the park. When we take off a gust of wind blows my hair back and sends chills all down my back, through the entire ride there was a swarm of butterflies in my stomach that slowly faded away as we creeped closer to the end. Out of breath I ran to the next ride with my family, and being tired did not slow any of us down. We stop at the next ride called, Outlaw Run. I have heard all good things about the ride, which probably would explain why the line is so long. Disregarding the line, the building is very western; there is old cowboy boots hanging on the wall along with the workers joining the western mood by talking in accents. I finally made it to the front of the line and the anticipation had been killing me to ride this. I hop in the seat and hear the hiss, as the harness comes down onto my torso and pushes against me. The workers came by and made sure I was secure before we started the ride. My heart was pounding out of my chest as we inch closer and closer to the top of the hill. I look down at the beautiful view of the lake with the Christmas lights reflecting off of it. But the view didnt last long until†¦ we dropped! This day has been unimagi nable and I couldnt think of how it could get any better, but it was starting to get darker and more and more lights were turned on; before it got too late we wanted to stay for the night show. The night show is down in Echo Hollow, where some of the bestShow MoreRelatedWhat Organizations, Ideologies, and Leaders Did Africans Create and Engage in the 20th Century to Promote and Advance Their Liberation?1220 Words   |  5 PagesWhat organizations, ideologies, and leaders did Africans create and engage in the 20th century to promote and advance their liberation? [Abstract] The 20th century composed of many organizations ideologies and leaders helping to promote and advance liberation both in America and worldwide. 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